The comparative histological studies of the testis in the wild and cultured African catfish species (Clarias gariepinus)
2012
Agbebi, O.T. | Ademolu, O.M. | Adebayo, A.O.
The comparative study of the structure of testes of both the cultured and wild Male Clarias species was carried out with the aim of highlighting the possible differences in the testicular structure between them. The data were analysed based on the Regression and Correlation Analysis to examine the relationship between and within the data using Microsoft Excel data analysis tool. The Samples of the testes were fixed in Bouin's fluid for 72 hours. The tissue samples were passed through graded concentrations of alcohol at 50%, 70%, 90%, and absolute alcohol (100%) in order to achieve dehydration. The tissues were cleared in two jars of xylene for one hour and then embedded after passing through the four changes of paraffin wax at 60~'C. Paraffin sections of 5. Micron thick were obtained on a microtome. These were mounted on clear albuminized slides after floating on a warm water bath and then dried in an oven and stained with Haematoxylin and Eosin (H & E). At the same time, some of the cut sections of the testes were stained with Periodic Acid Schiff (PAS) reagents. All the slides were examined under the light microscope. On the slides stained with H&E and PAS, the histological features of the testis were evaluated and the histomorphometry of the testes determined. The findings of this work showed that there was no histological difference in the testicular structure of both domestically reared and wild strains of Clarias gariepinus species. At the end of the experiment, this work showed that testicular structure of Clarias gariepinus in the cultured and the wild are histologically similar.
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