Cytological approaches combined with chemical analysis reveals the layered nature of flax mucilage
2019
Miart, Fabien | Fournet, Françoise | Dubrulle, Nelly | Petit, Emmanuel | Demailly, Hervé | Dupont, Loïc | Zabijak, Luciane | Marcelo, Paulo | Boudaoud, Arezki | Pineau, Christophe | Guenin, Stéphanie | van Wuytswinkel, Olivier | Mesnard, François | Pageau, Karine | Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI) ; Université de Picardie Jules Verne (UPJV) | Institut National de la Recherche Agronomique (INRA) | Centre de ressources régionales en biologie moléculaire - UPJV (CRRBM) ; Université de Picardie Jules Verne (UPJV) | Laboratoire réactivité et chimie des solides - UMR CNRS 7314 UPJV (LRCS) ; Advanced Lithium Energy Storage Systems - ALISTORE-ERI (ALISTORE-ERI) ; Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS) | Plateforme d’Ingénierie Cellulaire et Analyses des Protéines - UPJV (ICAP) ; Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS) | Reproduction et développement des plantes (RDP) ; École normale supérieure de Lyon (ENS de Lyon) ; Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Centre National de la Recherche Scientifique (CNRS) | Universite of Picardie Jules Verne (UPJV); French Government ANR-001; French Government [COST action] FA1006 | ANR-10-IEED-0001,PIVERT,Picardie Innovations Végétales, Enseignement et Recherches Technologiques(2010)
International audience
Show more [+] Less [-]English. The external seed coat cell layer of certain species is specialized in the production and extrusion of a polysaccharide matrix called mucilage. Variations in the content of the released mucilage have been mainly associated with genetically regulated physiological modifications. Understanding the mucilage extrusion process in crop species is of importance to gain deeper insight into the complex cell wall biosynthesis and dynamics. In this study, we took advantage of the varying polysaccharide composition and the size of the flax mucilage secretory cells (MSCs) to study mucilage composition and extrusion in this species of agricultural interest. We demonstrate herein that flax MSCs are structured in four superimposed layers and that rhamnogalacturonans I (RG I) are firstly synthesized, in the upper face, preceding arabinoxylan and glucan synthesis in MSC lower layers. Our results also reveal that the flax mucilage release originates from inside MSC, between the upper and deeper layers, the latter collaborating to trigger polysaccharide expansion, radial cell wall breaking and mucilage extrusion in a peeling fashion. Here, we provide evidence that the layer organization and polysaccharide composition of the MSCs regulate the mucilage release efficiency like a peeling mechanism. Finally, we propose that flax MSCs may represent an excellent model for further investigations of mucilage biosynthesis and its release.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Institut national de la recherche agronomique