Chemical inhibitors of monogalactosyldiacylglycerol synthases in Arabidopsis thaliana.
2011
Botté, Cyrille y | Deligny, Michael | Roccia, Aymeric | Bonneau, Anne-Laure | Saïdani, Nadia | Hardré, Hélène | Aci, Samia | Yamaryo-Botté, Yoshiki | Jouhet, Juliette | Dubots, Emmanuelle | Loizeau, Karen | Bastien, Olivier | Brehelin, Laurent | Joyard, Jacques | Cintrat, Jean-Christophe | Falconet, Denis | Block, Maryse A | Rousseau, Bernard | Lopez, Roman | Maréchal, Eric | Laboratoire de physiologie cellulaire végétale (LPCV) ; Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) ; Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) | Institut de Biologie et de Technologies de Saclay (IBITECS) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay | Dynamique des interactions membranaires normales et pathologiques (DIMNP) ; Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) | Centre de biophysique moléculaire (CBM) ; Université d'Orléans (UO)-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | Méthodes et Algorithmes pour la Bioinformatique (MAB) ; Laboratoire d'Informatique de Robotique et de Microélectronique de Montpellier (LIRMM) ; Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) | This work was supported by ANR-05-EMPB-017-01, ANR-06-MDCA-014, ANR-10-BLAN-1524-01 and ANR-2010-JCJC-1606-01 grants from the Agence Nationale de la Recherche, France. C.Y.B. was supported by a Marie Curie International Outgoing Fellowship action from the European Commission. | European Project:
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Show more [+] Less [-]English. Monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) are the main lipids in photosynthetic membranes in plant cells. They are synthesized in the envelope surrounding plastids by MGD and DGD galactosyltransferases. These galactolipids are critical for the biogenesis of photosynthetic membranes, and they act as a source of polyunsaturated fatty acids for the whole cell and as phospholipid surrogates in phosphate shortage. Based on a high-throughput chemical screen, we have characterized a new compound, galvestine-1, that inhibits MGDs in vitro by competing with diacylglycerol binding. Consistent effects of galvestine-1 on Arabidopsis thaliana include root uptake, circulation in the xylem and mesophyll, inhibition of MGDs in vivo causing a reduction of MGDG content and impairment of chloroplast development. The effects on pollen germination shed light on the contribution of galactolipids to pollen-tube elongation. The whole-genome transcriptional response of Arabidopsis points to the potential benefits of galvestine-1 as a unique tool to study lipid homeostasis in plants.
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