A canine adenovirus type 2 vaccine vector confers protection against foot-and-mouth disease in guinea pigs
2018
de Vleeschauwer, Annebel R. | Zhou, Xiaocui | Lefebvre, David J. | Gamier, Annabelle | Watier, Fleur | Pignon, Charly | Lacour, Sandrine | Zientara, Stéphan | Bakkali, Labib | de Clercq, Kris | Klonjkowski, Bernard | Sciensano [Bruxelles] ; Pasteur Network (Réseau International des Instituts Pasteur) | Institut National de la Recherche Agronomique (INRA) | China Animal Health and Epidemiology Center | Virologie UMR1161 (VIRO) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | École nationale vétérinaire d'Alfort (ENVA) | Belgian Federal Public Service for Health, Food Chain Safety and Environment [RI14/2-26]; French Agence Nationale de la Recherche [ANR-14-ANWA-0007-01] | ANR-14-ANWA-0007,TRANSCRIPTOVAC,Host response gene signatures associated with FMDV infection, vaccination and persistence.(2014) | European Project: 226556,EC:FP7:KBBE,FP7-KBBE-2008-2B,FMD-DISCONVAC(2009)
International audience
Show more [+] Less [-]English. Vaccination is a key element in the control of foot-and-mouth disease (FMD). The majority of the antigenic sites that induce protective immune responses are localized on the FMD virus (FMDV) capsid that is formed by four virus-encoded structural proteins, VP1 to VP4. In the present study, recombinant canine adenovirus type 2 (CAV2)-based FMD vaccines, Cav-Pl/3C R degrees and Cav-VP1 R degrees, respectively expressing the structural P1 precursor protein along with the non-structural 3C protein or expressing the structural VP1 protein of the FMDV strain O/FRA/1/2001, were evaluated as novel vaccines against FMD. A strong humoral immune response was elicited in guinea pigs (GP) following immunization with Cav-P1/3C R, while administration of Cav-VP1 R degrees did not induce a satisfying antibody response in GP or mice. GP were then used as an experimental model for the determination of the protection afforded by the Cav/P1 R degrees vaccine against challenge with the FMDV strain O-1 Manisa/Turkey/1969. The Cav-P1/3C R vaccine protected GP from generalized FMD to a similar extent as a high potency double-oil emulsion O-1 Manisa vaccine. The results of the present study show that CAV2-based vector vaccines can express immunogenic FMDV antigens and offer protection against generalized FMD in GP. This suggest that Cav-P1 R FMDV vaccine may protect natural host species from FMD. In combination with an appropriate diagnostic test, the Cav-P1 R degrees FMDV vaccine may also serve as a marker vaccine to differentiate vaccinated from infected animals.
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