Specificity of thioredoxins and glutaredoxins as electron donors to two distinct classes of Arabidopsis plastidial methionine sulfoxide reductases B
2007
Vieira dos Santos, Christina | Laugier, Edith | Tarrago, Lionel | Massot, Vincent | Issakidis-Bourguet, Emmanuelle | Rouhier, Nicolas | Rey, Pascal | Commissariat à l'énergie atomique et aux énergies alternatives (CEA) | Biodiversité et Biotechnologie Fongiques (BBF) ; Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM) | Institut de biotechnologie des plantes (IBP) ; Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) | Interactions Arbres-Microorganismes (IAM) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM) ; Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
International audience
Show more [+] Less [-]English. Methionine sulfoxide reductases (MSRs) A and B reduce methionine sulfoxide (MetSO) S‐ and R‐diastereomers, respectively, back to Met using electrons generally supplied by thioredoxin. The physiological reductants for MSRBs remain unknown in plants, which display a remarkable variety of thioredoxins (Trxs) and glutaredoxins (Grxs). Using recombinant proteins, we show that Arabidopsis plastidial MSRB1 and MSRB2, which differ regarding the number of presumed redox‐active cysteines, possess specific reductants. Most simple‐module Trxs, especially Trx m1 and Trx y2, are preferential and efficient electron donors towards MSRB2, while the double‐module CDSP32 Trx and Grxs can reduce only MSRB1. This study identifies novel types of reductants, related to Grxs and peculiar Trxs, for MSRB proteins displaying only one redox‐active cysteine.
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