Construction of a contig of BAC clones spanning the region of the apple scab avirulence gene AvrVg
2007
Broggini, G. A. L. | Le Cam, Bruno | Parisi, Luciana, L. | Wu, Chih-Hang | Zhang, H.B. | Gessler, Cesare | Patocchi, Andrea | Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich) | Unité de recherche Pathologie végétale et phytobactériologie ; Institut National de la Recherche Agronomique (INRA) | Department of Soil and Crop Sciences ; Texas A&M University System
Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699
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Show more [+] Less [-]English. The ascomycete Venturia inaequalis, causal pathogen of apple scab, underlies a gene-for-gene relationship with its host plant apple (Malus spp.). 'Golden Delicious', one of the most common cultivated apples in the world, carries the ephemeral resistance gene Vg. Avirulence gene AvrVg, matching resistance gene Vg has recently been mapped on the V. inaequalis genome. In this paper, we present the construction of a BAC library from a V. inaequalis AvrVg isolate. The library is composed of 7680 clones, with an average insert size of 80 kb. By hybridization, it has been estimated that the library contains six haploid genome equivalents. Thus the V. inaequalis genome can be predicted to be approximately 100 Mb in size. A chromosome walk, starting from the marker VirQ5 co-segregating with AvrVg, has been performed using the BAC library. Twelve BAC clones were identified during four steps of the chromosome walking. The size of the resulting contig is approximate to 330 kb
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