Sequence and polymorphism analysis of the camel (Camelus dromedarius) myostatin gene
2015
Muzzachi, Stefania | Oulmouden, Ahmad | Cherifi, Youcef | Yahyaoui, Mohamed Habib | Zayed, Mohamed | Burger, Pamela | Lacalandra, Giovanni Michele | Faye, Bernard | Ciani, Elena | Department of Biosciences, Biotechnology and Biopharmaceutics ; Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA) | Unité de Génétique Moléculaire Animale (UMR GMA) ; Université de Limoges (UNILIM)-Institut National de la Recherche Agronomique (INRA) | Département de Génétique Moléculaire Appliquée, Faculté des Sciences de la Nature, Laboratoire de Génétique Moléculaire et Cellulaire ; Université des sciences et de la Technologie d'Oran Mohamed Boudiaf [Oran] (USTO MB) | Laboratoire d'élevage et de la Faune Sauvage ; Institut des Régions Arides (IRA) | Department of Animal Breeding, Meat Quality and Safety ; Desert Research Centre (DRC) | Institute of Population Genetics ; University of Veterinary Medicine [Vienna] (Vetmeduni) | Department of Emergency and Organ Transplantation, Section of Animal Production ; Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA) | Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | MOA Camel Project ; Food and Agriculture Organization of the United Nations [France] (FAO) ; Food and Agriculture Organization of the United Nations [Rome, Italie] (FAO)-Food and Agriculture Organization of the United Nations [Rome, Italie] (FAO) | European Union within the ENPI-CBC-MED I.B/1.1/493
Myostatin (MSTN), a negative regulator of skeletal muscle development in mammals, represents a key target for genetic investigations in meat-producing animals, with mutations responsible for increased skeletal-muscle mass currently described in several livestock species. Dromedary camels play a major economic role as suppliers of meat for human consumption across several countries. Notwithstanding, a comprehensive characterization of the sequence variability at the Camelus dromedarius MSTN locus was still lacking. Here we present the first extensive sequence and polymorphism analysis of the MSTN gene in the Camelus dromedarius species. Out of more than 3.6 kb of nucleotide sequence screened on 22 animals from 3 different Northern African regions, only 3 variant sites in the first intron were detected. The low observed diversity may reflect the evolutionary history of the species, likely developed as domesticates from a low variable wild ancestor population. Sequence identity among Camelus dromedarius and other Cetartiodactyla highlighted a tree topology consistent with previous reports of a closer relationship between Tylopoda and Suiformes. A close similarity between C. ferus and Camelus dromedarius was observed within Tylopoda. A markedly higher sequence identity between Camelus dromedarius and the other vertebrate species was observed at the MSTN locus compared to other genes, thus confirming it as a highly conserved target across mammals.
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