Evaluation of the occurrence of sporulating and nonsporulating pathogenic bacteria in manure and in digestate of five agricultural biogas plants

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English. Abstract The number of agricultural biogas plants has been increasing in the past decades in some European countries. Digestates obtained after anaerobic digestion (AD) of manure are usually spread on agricultural land; however, their hygiene status regarding pathogens posing public health and/or animal health challenges has been poorly characterized up to now in France. In this study, three replicates of manure and digestate were collected from five farm biogas plants receiving animal manure in order to assess the occurrence and concentrations of sporulating ( Clostridium botulinum , Clostridioides difficile , Clostridium perfringens ) and nonsporulating ( Listeria monocytogenes , thermotolerant Campylobacter spp., Salmonella , Escherichia coli , enterococci) bacteria. Concentrations of E. coli , enterococci, and C. perfringens in digestates ranged from 10 2 to 10 4 , 10 4 to 10 5 , and <10 3 to 7 × 10 5 CFU/g, respectively. Salmonella and C. difficile were detected in manure and digestate from the five biogas plants at concentrations ranging from <1.3 to >7 × 10 2 MPN/g and from 1.3 to 3 × 10 2 MPN/g, respectively. Thermotolerant Campylobacter , detected in all the manures, was only found in two digestates at a concentration of cells ranging from <10 to 2.6 × 10 2 CFU/g. Listeria monocytogenes and C. botulinum were detected in three manures and four digestates. The bacterial counts of L. monocytogenes and C. botulinum did not exceed 3 × 10 2 and 14 MPN/g, respectively. C. botulinum type B was detected at very low level in both the manure and digestate of farm biogas plants with no botulism history. The levels of pathogenic bacteria in both manure and digestate suggested that some bacteria can persist throughout AD.