Extracellular protease characterization of Lactococcus lactis subsp. lactis and its application on protein hydrolysis toward Indonesian sea cucumber(Holothuria sp.) and Lorjuk shelfish (Solen sp.) as an antioxidant alternative
2023
Sari, S.S. | Mustopa, A.Z. | Irawan, H. | Rohmah, I.A. | Zunita, N.L.A.A. | Hafiludin | Chandra, A.B. | Putranto, W.S.
Enzymatic hydrolysis is more efficient compared to other types of protein hydrolysis methods due to the fact that enzymes function as specific catalysts that can produce peptides in a less complex form and avoid non-hydrolytic changes. Extracellular protease from Lactococcus lactis subsp. lactis (LAC3) has the potential as an agent for enzymatic hydrolysis of marine products, especially for non-fibrillar and non-collagen protein types. Extracellular protease from LAC3 was successfully isolated and was able to hydrolyze sea cucumber and lorjuk shellfish. Hydrolyzed products appear to have molecular weights of 4.6 and 15 kDa. The hydrolysates from the enzymatic hydrolysis of the two marine samples have significant antioxidant activities based on the DPPH inhibition assay. The best 3D protein structure modeling was done using the AlphaFold web server.
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