Persistence of fluoroquinolones and ciprofloxacin resistant Enterobacteriacae in soil after poultry manure application
2015
Dabert, P. | Pourcher, A.M. | Jadas Hécart, A. | Cotinet, P. | Ziebal, C. | Le Roux, S. | Moraru, R. | Kempf, Isabelle | Optimisation des procédés en Agriculture, Agroalimentaire et Environnement (UR OPAALE) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Littoral, Environnement, Télédétection, Géomatique UMR 6554 (LETG) ; Université de Caen Normandie (UNICAEN) ; Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-École Pratique des Hautes Études (EPHE) ; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut de Géographie et d'Aménagement Régional de l'Université de Nantes (IGARUN) ; Université de Nantes (UN)-Université de Nantes (UN) | Chambre Régionale d'Agriculture de Bretagne | UNIVERSITATEA DE STIINTE AGRICOLES IASI ROM ; Partenaires IRSTEA ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Laboratoire de Ploufragan - Plouzané ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED
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Show more [+] Less [-]English. This study aimed to evaluate the persistence (i) of two fluoroquinolones, enrofloxacin (ENR) and ciprofloxacin (CIP), and (ii) of CIP-resistant Enterobacteriaceae in soil after poultry manure application. The amounts of ENR and CIP in soil did not significantly change over the 48 day- experimental period. CIP (≤ 30 μg kg-1) was detected in weaker concentrations than ENR (20 to 250 μg kg-1 of soil). The concentration of Enterobacteriaceae (102-103 CFU g-1) did not significantly decrease until Day 36. A total of 145 isolates were identified as belonging to E. coli and to 6 genera. The ciprofloxacin MIC of E. coli reached 32 mg L-1 whereas the other strains of Enterobacteriaceae had a MIC ≤ 0.25 mg L-1. The 71 strains of E. coli were classified in 12 ERIC-PCR genotypes. One genotype, corresponding to a resistant strain, was detected until Day 89. This study showed that enrofloxacin and CIP-resistant E. coli persisted at least 48 and 89 days, respectively, in soil. However, their presence did not increase the MIC of Enterobacteriaceae originating from soil.
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