Expression of VP7, a Bluetongue virus group specific antigen by viral vectors: Analysis of the induced immune responses and evaluation of protective potential in sheep
2014
Bouet Cararo, Coraline | Contreras, Vanessa | Caruso, Agathe | Top, Sokunthea | Szelechowski, Marion | Bergeron, Corinne | Viarouge, Cyril | Desprat, Alexandra | Relmy, Anthony | Guibert, Jean-Michel | Dubois, Eric | Thiéry, Richard | Breard, Emmanuel | Bertagnoli, Stéphane | Richardson, Jennifer | Foucras, Gilles | Meyer, Gilles, G. | Schwartz-Cornil, Isabelle | Zientara, Stéphan | Klonjkowski, Bernard | Virologie UMR1161 (VIRO) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) ; Institut National de la Recherche Agronomique (INRA) | Interactions hôtes-agents pathogènes [Toulouse] (IHAP) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | Centre de Physiopathologie Toulouse Purpan (CPTP) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) | Institut National de la Santé et de la Recherche Médicale (INSERM) | Unité de pathologie des petits ruminants ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | UE Network of Excellence, EPIZONE FOOD-CT-2006-016236 | European Project: 245266,EC:FP7:KBBE,FP7-KBBE-2009-3,ORBIVAC(2010)
International audience
Show more [+] Less [-]English. Bluetongue virus (BTV) is an economically important Orbivirus transmitted by biting midges to domestic and wild ruminants. The need for new vaccines has been highlighted by the occurrence of repeated outbreaks caused by different BTV serotypes since 1998. The major group-reactive antigen of BTV, VP7, is conserved in the 26 serotypes described so far, and its role in the induction of protective immunity has been proposed. Viral-based vectors as antigen delivery systems display considerable promise as veterinary vaccine candidates. In this paper we have evaluated the capacity of the BTV-2 serotype VP7 core protein expressed by either a non-replicative canine adenovirus type 2 (Cav-VP7 R-0) or a leporipoxvirus (SG33-VP7), to induce immune responses in sheep. Humoral responses were elicited against VP7 in almost all animals that received the recombinant vectors. Both Cav-VP7 R-0 and SG33-VP7 stimulated an antigen-specific CD4(+) response and Cav-VP7 R-0 stimulated substantial proliferation of antigen-specific CD8(+) lymphocytes. Encouraged by the results obtained with the Cav-VP7 R-0 vaccine vector, immunized animals were challenged with either the homologous BTV-2 or the heterologous BTV-8 serotype and viral burden in plasma was followed by real-time RT-PCR. The immune responses triggered by Cav-VP7 R-0 were insufficient to afford protective immunity against BTV infection, despite partial protection obtained against homologous challenge. This work underscores the need to further characterize the role of BTV proteins in cross-protective immunity.
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