Alkali-peroxide treatment of sugar cane bagasse : Effect of chemical charges on the efficiency of xylan isolation and susceptibility of bagasse to saccharification
2022
Solier, Yamil Nahún | Mocchiutti, Paulina | Cabrera, María Noel | Saparrat, Mario Carlos Nazareno | Zanuttini, Miguel Ángel Mario | Inalbon, María Cristina
Sugar cane is a promising raw material for the extraction of hemicelluloses. An alkali treatment of sugar cane bagasse under proper conditions followed by a precipitation using a reasonable quantity of ethanol can be an effective method to isolate these polysaccharides. In this work, bagasse is treated to obtain two products: (a) polymeric hemicellulose and (b) an enzymatic hydrolysate from the treated bagasse after hemicellulose extraction. The effects of charges of sodium hydroxide (10, 20, 40% w/w) and hydrogen peroxide (0, 10, 20% w/w) in the alkali treatment were evaluated. A 3² experimental design was considered under control of metal ions and inert atmosphere during alkaline treatment. An acceptable proportion of xylose plus arabinose could be extracted from bagasse (up to 18.4 g/100 g of bagasse). Both the alkali and peroxide showed strong effects on the yield of the extraction, as well as on the yield of the precipitation in ethanol-water solution. Besides, the susceptibility of bagasse for cellulolytic enzymatic hydrolysis is improved. The highest hemicellulose precipitation yield was 85% which corresponded to the treatment with the highest alkali charge without peroxide. The highest yield of enzymatic hydrolysis was obtained for the highest alkali and peroxide charges.
Show more [+] Less [-]Instituto de Fisiología Vegetal
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