Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans
2006
Burton, R. | Wilson, S. | Hrmova, M. | Harvey, A. | Shirley, N. | Medhurst, A. | Stone, B. | Newbigin, E. | Bacic, A. | Fincher, G.
A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-ß-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-ß-D-glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-ß-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-ß-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-ß-D-glucan biosynthesis.
Show more [+] Less [-]Rachel A. Burton, Sarah M. Wilson, Maria Hrmova, Andrew J. Harvey, Neil J. Shirley, Anne Medhurst, Bruce A. Stone, Edward J. Newbigin, Antony Bacic, Geoffrey B. Fincher
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