Molecular discrimination of garfish Hyporhamphus (Beloniformes) larvae in southern Australian waters
2001
Noell, C. | Donnellan, S. | Foster, R. | Haigh, L.
A multiplex polymerase chain reaction (PCR) assay developed for discrimination between garfish larvae (family Hemiramphidae, order Beloniformes) found in southern Australian waters was based on species-specific amplification of part of the mitochondrial control region. The species were easily discerned by the number and distinct sizes of PCR products (Hyporhamphus melanochir, 443 bp; H. regularis, 462 and 264 bp). Although based on a single gene, the method will correctly identify the species of individuals in at least 96% of tests for H. melanochir and 94% of tests for H. regularis.
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