Distinct strains of the re‐emergent Cassava common mosaic virus (Genus: Potexvirus) infecting cassava in Argentina
2018
Zanini, A.A | Cuéllar, Wilmer Jose | Celli, M.G. | Luque, V.A. | Medina, R. | Conci, V.C. | Di Feo, L.V. del
Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV) was identified as a mechanically transmitted potexvirus (Fam. Alphaflexiviridae). In Argentina, cassava is grown mainly in the North East region (NEA) that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated to severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In this work we successfully purified the virus and developed a double-antibody sandwich (DAS) ELISA test from an Argentinean field-isolate of CsCMV, to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate cluster was formed by sequences isolates from NEA samples which only share 77.1 to 80.3% nucleotide identity with the other clusters. These results indicated the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.
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