Chemistry of developing bordered-pit rims in balsam-fir trees
2016
Savidge, Rodney A.
Rims of bordered pits form on primary walls of radially enlarged cambial derivatives prior to the onset of general secondary-wall formation. A recent report (Botany, 2014, 92(7): 495-511) raised the possibility that the chemical composition of the rim might be different from that of the secondary wall. To investigate this, early-stage non-fluorescent and late-stage autofluorescent rims were separated from cambial derivatives of Abies balsamea (L.) Mill. and purified to homogeneity by density-gradient centrifugation. Solid state nuclear magnetic resonance spectroscopy, Raman microsspectroscopy, combined gas chromatography - mass spectroscopy, enzyme digestion and chemical resilience data support the interpretation that cellulose alone is the microfibrillar polysaccharide of non-fluorescent early-stage rims. A lignin is additionally present in late-stage rims, but it evidently bonds with cellulose because rims are extraordinarily resistant to hydrolysis by either enzymes or strong acid.
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