Quantification of vitamin A in palm oil using a fast and simple portable device method validation and comparison to high-performance liquid chromatography
2011
Rohner, Fabian | Frey, Simone K. | Mothes, Ralf | Hurtienne, Andrea | Hartong, Simone | Bosso, Patrice Emery | Bui, Mai | Schweigert, Florian J. (Prof. Dr.) | Northrop-Clewes, Christine
Vitamin A deficiency continues to be a global public health problem. Fortification of oil with vitamin A is considered a cost-effective, feasible strategy to prevent this problem but quality control poses a challenge to program implementation. To overcome this, we have validated a newly developed device that quantitatively measures the content of retinyl palmitate in refined palm oil, is simple to use, and yields immediate results. Linearity of analysis rand from 2.5-30 mg retinol equivalents (RE)/kg of palm oil, with 2.5 mg RE/kg being the determination limit; inter- and intra-assay precision ranged from 1.4-7.1 To. Comparison with a high-performance Liquid chromatography method showed high agreement between the methods (R-2 = 0.92; Limits of Agreement: -1.24 mg to 2.53 mg RE/kg), and further comparisons illustrate that the new device is useful in low resource settings. This device offers a field- and user-friendly solution to quantifying the vitamin A content in refined palm oil.
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