Combined stereomicroscope and SEM disentangle the fine morphology of the undescribed larva and puparium of the hoverfly Milesia crabroniformis (Fabricius, 1775) (Diptera: Syrphidae)

With over 80 species, Milesia Latreille, 1804 is a hoverfly genus (Diptera: Syrphidae) is found in all continents except for Australia and the Antarctica. However, little is known about its life cycle and biology. The three Milesia species for which early stages are known have saproxylic larvae, suggesting that the larvae of all other Milesia species are also saproxylic. The early stages of the three Milesia species occurring in Europe are undescribed. Milesia crabroniformis (Fabricius, 1775), a mimic of the hornet Vespa crabro Linnaeus, 1758, is the largest hoverfly in Europe and is listed as Least Concern in the IUCN European Red List of Hoverflies. We here report the first early stages of Milesia ever found in Europe, describing them and their breeding sites. Larvae of M. crabroniformis were collected in water-filled tree holes of live chestnut trees (Castanea sativa Mill.) in Málaga, Southern Spain in 2020-2021. Various studies based on stereomicroscope and scanning electron microscopy (SEM) techniques have proven useful in diagnosing hoverfly early stages by the observation of their fine morphology. Thus, these techniques were also used here to characterize the second (L2) and third (L3) stage larvae of M. crabroniformis, as well as the puparium. A Leica M205 C binocular stereomicroscope and a Jeol JSM-ITH500HR SEM were used. The head skeleton and chaetotaxy of the L3 larva were described and illustrated. Adjustments to the diagnosis of the larvae of Milesia are proposed based on the number of hooks from the primary row of the main group of hooks. The new early stages are compared with those of other Milesia hoverflies, as well as with those of the sister group Spilomyia Meigen, 1803. The knowledge of the larval biology and breeding sites of saproxylic insects is useful for implementing forest management measures and species’ conservation programs.

This work belongs to the ‘Fauna Ibérica’ Project (PGC2018-095851-A-C65) of the Spanish Ministry of Science, Innovation and Universities. Antonio Ricarte’s position (UATALENTO17-18) at the University of Alicante is funded by the “Vicerrectorado de Investigación y Transferencia del Conocimiento”. Student grant of José Orengo’s was funded by the University of Alicante (UAFPU2019B-57). Javier Quinto’s position were funded by Andalusian Regional Government (PP.PEI.IDF2019.001) and the Spanish Ministry of Science and Innovation (PID2020-115140RB-I00). This work is part of a PhD thesis of the first author.