GARP transcription factors repress Arabidopsis nitrogen starvation response via ROS-dependent and -independent pathways
2021
Safi, Alaeddine | Medici, Anna | Szponarski, Wojciech | Martin, Florence | Clément-Vidal, Anne | Marshall-Colon, Amy | Ruffel, Sandrine | Gaymard, Frédéric | Rouached, Hatem | Leclercq, Julie | Coruzzi, Gloria | Lacombe, Benoît | Krouk, Gabriel, G. | Biochimie et Physiologie Moléculaire des Plantes (BPMP) ; Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Department of Plant Systems Biology, VIB, and Department of Plant Biotechnology and Bioinformatics ; Universiteit Gent = Ghent University = Université de Gand (UGENT) | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Center for Genomics and Systems Biology ; Department of Biology [New York] ; New York University [New York] (NYU) ; NYU System (NYU)-NYU System (NYU)-New York University [New York] (NYU) ; NYU System (NYU)-NYU System (NYU) | ANR-14-CE19-0008,IMANA,Identification de régulations moléculaires majeures impliquées dans l'adaptation des plantes à la disponibilité en azote(2014)
International audience
Show more [+] Less [-]English. Abstract Plants need to cope with strong variations of nitrogen availability in the soil. Although many molecular players are being discovered concerning how plants perceive NO3− provision, it is less clear how plants recognize a lack of nitrogen. Following nitrogen removal, plants activate their nitrogen starvation response (NSR), which is characterized by the activation of very high-affinity nitrate transport systems (NRT2.4 and NRT2.5) and other sentinel genes involved in N remobilization such as GDH3. Using a combination of functional genomics via transcription factor perturbation and molecular physiology studies, we show that the transcription factors belonging to the HHO subfamily are important regulators of NSR through two potential mechanisms. First, HHOs directly repress the high-affinity nitrate transporters, NRT2.4 and NRT2.5. hho mutants display increased high-affinity nitrate transport activity, opening up promising perspectives for biotechnological applications. Second, we show that reactive oxygen species (ROS) are important to control NSR in wild-type plants and that HRS1 and HHO1 overexpressors and mutants are affected in their ROS content, defining a potential feed-forward branch of the signaling pathway. Taken together, our results define the relationships of two types of molecular players controlling the NSR, namely ROS and the HHO transcription factors. This work (i) up opens perspectives on a poorly understood nutrient-related signaling pathway and (ii) defines targets for molecular breeding of plants with enhanced NO3− uptake.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Institut national de la recherche agronomique