Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities
2021
Sahli, Célia | Moya, Sergio | Lomas, John | Gravier-Pelletier, Christine | Briandet, Romain | Hémadi, Miryana | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | ANR-10-LABX-0096,SEAM,Science and Engineering for Advanced Materials and devices(2010)
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Show more [+] Less [-]English. The behavior of Listeria monocytogenes communities in the food chain is closely associated with their spatial organization. Whether as biofilms on industrial surfaces or as microcolonies in food matrices, the resulting physiological diversification combined with the presence of extracellular polymeric substances (EPS) triggers emergent community functions involved in the pathogen survival and persistence (e.g., tolerance to dehydration, biocides, or preservatives). In this contribution, we present a noninvasive confocal laser microscopy (CLM) protocol allowing exploration of the spatial organization of L. monocytogenes communities on various inert or nutritive materials relevant for the food industry.
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