Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae
2007
Nerland, Audun H. | Skår, Cecilie Kristin | Eriksen, Tove Boge | Bleie, Hogne
Journal home page: http://www.int-res.com/journals/dao/
Show more [+] Less [-]We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 × 104 TCID50 (50% tissue culture infectious dose) ml–1. Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 × 107 virus particles ml–1 seawater.
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