Isolation, identification and evaluation of bacteriocin activity of lactic acid bacteria from intestinal bacterial flora of Planiliza klunzingeri (Day, 1888)
2023
Fatemeh Shayesteh
The study delved into bacteriocins, peptides with antibacterial properties, originating from select lactic acid bacteria. Isolating 18 such bacteria from Liza klunzingeri's intestine, their potential in bacteriocin production against food pathogens was assessed. Crude bacteriocins from 7 isolates exhibited the ability to inhibit food pathogens via an agar spot test. Notably, bacteriocins derived from LK5 and LK12 isolates displayed a broader inhibitory spectrum against the pathogens under study. Further analysis revealed that the studied bacteriocins were susceptible to proteolytic enzymes, leading to complete degradation. However, these bacteriocins retained their antibacterial potency when subjected to diverse detergents, pH variations, and temperature fluctuations. Genetic analysis via the 16S rRNA gene identified LK5 and LK12 as strains of Lactobacillus plantarum, with similarity percentages of 98.55% and 99.46%, respectively. The findings highlight L. Klunzingeri's intestinal bacterial flora as a promising source for isolating bacteriocin-producing bacteria. Specifically, bacteriocins derived from L. Plantarum LK5 and L. Plantarum LK12 showcase potential as biological preservatives within the food industry, warranting further exploration.
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