Hop pellets as an interesting source of antioxidant active compounds
2013
Andrea Holubková | Silvia Mošovská | Ernest Šturdík | Barbora Baloghová
<!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG /> </o:OfficeDocumentSettings> </xml><![endif]--> <div style="mso-element: frame; mso-element-frame-width: 498.1pt; mso-element-frame-height: 306.05pt; mso-element-frame-vspace: 3.1pt; mso-element-wrap: around; mso-element-anchor-vertical: page; mso-element-anchor-horizontal: page; mso-element-left: 50.3pt; mso-element-top: 57.8pt; mso-height-rule: exactly;"><table style="height: 416px;" border="0" cellspacing="0" cellpadding="0" width="664" align="left"><tbody><tr><td style="padding-top: 3.1pt; padding-right: 0cm; padding-bottom: 3.1pt; padding-left: 0cm;" height="416" align="left" valign="top"><div style="mso-element: para-border-div; border: none; border-bottom: solid windowtext 2.25pt; padding: 0cm 0cm 1.0pt 0cm;"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">Hop is a plant used by humankind for thousands of years. <span class="hps">This plant</span> <span class="hps">is one</span> <span class="hps">of the main</span> <span class="hps">and</span> <span class="hps">indispensable</span> <span class="hps">raw materials</span> <span class="hps">for the beer production</span>. It is used for various dishes preparation in the cuisine. <span class="hps">Hop is also used</span> <span class="hps">to inhibit</span> <span class="hps">bacterial contamination</span>. The hop extracts are used <span class="hps">for</span> <span class="hps">its</span> <span class="hps">sedative</span>, <span class="hps">antiseptic</span> <span class="hps">and</span> <span class="hps">antioxidant properties in medicine, as a part of many phytopharmaceuticals</span>. <span class="hps">The present paper</span> <span class="hps">have focused</span> <span class="hps">on</span> <span class="hps">the</span> <span class="hps">extraction of</span> <span class="hps">polyphenolic</span> <span class="hps">compounds from</span> 4 samples of <span class="hps">hop</span> <span class="hps">pellets varieties of Aurora, Saaz, Lublin and Saphir, on the analyzing of bioactive substances (polyphenolics and flavonoids) in prepared extracts and on the determination of antioxidant activity. <span style="mso-spacerun: yes;"> </span>The highest content of polyphenolic substances was determined in the sample Lublin (</span></span><span style="font-size: 10.0pt; color: black;">153.06 mg gallic acid (GAE)/g</span><span style="color: black;">) </span><span style="font-size: 10.0pt; color: black;">and Saaz (151.87 mg GAE/g). </span><span style="font-size: 10.0pt; color: black; mso-ansi-language: EN-US;" lang="EN-US">The amount of flavonoids in the samples <span style="mso-spacerun: yes;"> </span>was descending order Saaz > Saphir > Aurora > Lublin. Hops, as plant, is known by high content of antioxidant active substances. Antioxidant activity was determined using three independent spectrofotometric methods, </span><span style="font-size: 10.0pt;">radical scavenging assays using</span> <span style="font-size: 10.0pt;">2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical</span><span style="font-size: 10.0pt; color: black; mso-ansi-language: EN-US;"> </span><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">and ferric reducing antioxidant power (FRAP)<span style="color: black;">. The sample Aurora showed the highest ability to scavenge of ABTS radical cation. Antioxidant activity continued to decline in a row Saphir> Lublin> Saaz. The same trend was also observed by using the FRAP assay. The most effective DPPH radical scavengering activity had the sample </span></span><span style="font-size: 10.0pt; color: black;">Saaz a Saphir (p>0.05).<br /><br /><strong>doi:10.5219/270</strong><br /></span></div></td></tr></tbody></table></div> <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>SK</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:DontVertAlignCellWithSp /> 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