羊骨脂质的组成分析Lipid composition of sheep bones
2023
吕爽1，刘倩1，李红波1，张小勇2，徐丹1，苏从毅2，胡梁斌1，莫海珍1 LYU Shuang1, LIU Qian1, LI Hongbo1, ZHANG Xiaoyong2, XU Dan1, SU Congyi2, HU Liangbin1, MO Haizhen1
为了实现羊骨副产物的有效利用和为羊骨油高值化产品开发提供基础数据，以羊腿骨为原料，提取其中的油脂，分别采用高效液相色谱法（HPLC）、多维质谱鸟枪法（MDMS-SL）、气相色谱-质谱联用法（GC-MS）对羊骨油的甘油酯组成、脂质组学、脂肪酸组成进行分析。结果表明：羊骨油中甘油三酯含量达到96.76%，还有少量的甘油二酯（1.41%），而甘油一酯未检出；羊骨油中共鉴定出19种酰基肉碱、17种鞘磷脂、37种磷脂酰胆碱、39种甘油三酯，总含量分别为5.76、96.58、85.18 nmol/g和15.76 μmol/g；羊骨油中共鉴定出11种脂肪酸，其中饱和脂肪酸含量为11.51%， 单不饱和脂肪酸含量为72.34%，多不饱和脂肪酸含量为7.56%。 In order to realize the effective utilization of by-products sheep bones and provide basic data for the development of high-value sheep bone oil products, sheep leg bones were used as raw materials to extract oil, and the glyceride composition, lipidomics and fatty acid composition of sheep bone oil were analyzed by high performance liquid chromatography (HPLC), multidimensional mass spectrometry shotgun method (MDMS-SL) and gas chromatography-mass spectrometry (GC-MS) respectively. The results showed that the content of triglycerides in sheep bone oil reached 96.76%, and a small amount of diglycerides (1.41%) was identified, while no monoglycerides was detected. A total of 19 acylcarnitines, 17 sphingomyelins, 37 phosphatidylcholines and 39 triglycerides were identified in sheep bone oil, with total contents of 5.76, 96.58, 85.18 nmol/g and 15.76 μmol/g, respectively. 11 kinds of fatty acids including 11.51% saturated fatty acids, 72.34% monounsaturated fatty acids and 7.56% polyunsaturated fatty acids were detected in sheep bone oil.
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