Soil Aggregates and Aggregate-Associated Carbon and Nitrogen in Farmland in Relation to Long-Term Fertilization on the Loess Plateau, China

Soil aggregation plays a critical role in the maintenance of soil structure and crop productivity. Fertilization influences soil aggregation, especially by regulating soil organic carbon (SOC) and total nitrogen (TN) contents in aggregate fractions. Here, we conducted a fixed-site field experiment to quantify the effect of five N application rates: 0, 75, 150, 225, and 300 kg·N·ha⁻¹, denoted as N0, N75, N150, N225, and N300, respectively, on soil aggregate stability, aggregate-associated SOC and TN sequestration and crop productivity. Soil aggregates were divided into >0.25 (>5, 5–2, 2–1, 1–0.5, 0.5–0.25) and <0.25 mm through wet and dry sieving methods. The results showed that long-term fertilization increased the proportion of macro-aggregates (>0.25 mm), decreased the proportion of micro-aggregates (<0.25 mm), and improved the aggregates stability. Compared with N0, the proportion of micro-aggregates in N225 was significantly decreased by 66.45% under wet sieving, while the proportion of >5 mm macro-aggregates in N225 was significantly increased by 19.24% under dry sieving (<i>p</i> < 0.05). With the increase in N application rate, the bulk SOC and TN contents first increased and then decreased, and the SOC and TN of N225 were significantly increased by 17.75% and 72.33% compared with N0 (<i>p</i> < 0.05). More specifically, fertilization promoted the distribution and enrichment of SOC and TN in macro-aggregates and reduced the C/N of the micro-aggregates and the contribution of SOC and TN in the micro-aggregates. Compared with N0, the contribution rate of macro-aggregates to SOC and TN of N225 under wet sieving was significantly increased by 84.13 and 17.18%, respectively, while the C/N of micro-aggregates of N225 under wet and dry sieving methods was significantly decreased by 45.95 and 31.74%, respectively (<i>p</i> < 0.05). Moreover, fertilization improved the yield, and N225 significantly increased the total yield by 80.68% compared with N0 (<i>p</i> < 0.05). In conclusion, N225 was the suitable N application for improving soil aggregate stability, carbon and nitrogen sequestration, and crop productivity on the Loess Plateau, China.