Study on Influence Factors of Leaf Color of Aglaonema commutatum with Red Leaves in Tissue Culture
2022
Mengyun GU | Weida ZENG | Mingchi HUANG | Qinglian SU | Xiaomei FENG | Yanyan LIU | Xuelian ZHANG | Xiaoyun ZHOU
【Objective】Tissue culture is one of the important ways for rapid propagation of Aglaonema commutatum, the factors affecting leaf color in tissue culture were studied and explored to maintain the redness of leaf color, and to provide technical references for further stabilizing efficient tissue culture technology and regulating leaf color of A. commutatum with red leaves.【Method】Taking A. commutatum 'Guanghuahong' with red leaves as experimental material, the effects of proliferation medium, proliferation mode, light intensity and light quality on leaf color of A. commutatum were studied.【Result】The improved MS medium supplemented with 0.5 g/L K2SO4 was used for proliferation culture, and the proliferation and differentiation seedlings were more robust, with brighter leaf color, and the transplanting seedling rate was 90.00%.The leaf color of tissue culture seedlings cultured by the proliferation of clustered buds was more stable than that cultured by the proliferation of callus, and the transplanting seedling rate was higher. In the stages of proliferation and rooting, when the light intensity was 4 000 lx, the red plaque area increased, and the transplanting seedling rate was higher (88.89%). Under the light quality condition of red light : blue light = 3∶7, the lowest total content of chlorophyll in leaves was 0.037 mg/g, and the highest relative content of anthocyanin was 0.399 Units/g, and the leaves were red and brilliant.【Conclusion】Using the improved proliferation medium, adopting the proliferation mode of clustered buds, adjusting the light intensity and changing the red and blue light quality ratio can effectively maintain the redness of leaves and improve the production efficiency of tissue culture.
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