Rescate y germinación in vitro de embriones inmaduros de cedro negro (Juglans neotropica Diels) Germination and rescue in vitro of immature embryos of black cedar (Juglans neotropica Diels)
2012
Oscar Darío Quintero-García | Sonia Jaramillo-Villegas
Embriones inmaduros de cedro negro (Juglans neotropica) (Juglandaceae) con 16 y 20 semanas de desarrollo fueron removidos asépticamente de los frutos y sembrados durante 60 días en los medios de cultivo MS (Murashige y Skoog, 1962), WPM (Lloyd y McCown, 1980) y DKW (Driver y Kuniyuki, 1984), sin reguladores de crecimiento. Se observó que embriones con 16 semanas de desarrollo no germinaron en los tres medios de cultivo; por el contrario, los embriones con 20 semanas de desarrollo presentaron una germinación de 100%. Se encontró un marcado efecto del medio en el crecimiento de los embriones, siendo el medio MS mejor (P < 0.05) que los medios DKW y WPM, debido a que en él los embriones presentaron mayor altura y proporción tallo/raíz para la obtención de plántulas completas.<br>Immature embryos of Juglans neotropica (Juglandaceae) with 16 and 20 weeks of development were aseptically removed from the fruits and placed in different culture media MS (Murashige and Skoog, 1962), WPM (Lloyd and McCown, 1980) y DKW (Driver and Kuniyuki, 1984) without growth regulators for 60 days. It was observed that embryos with 16 weeks of development did not germinate in any of the three culture media. The embryos with 20 weeks of development germinated 100% in the three culture media. It was found a marked effect of the culture media on the growth of the embryos, the MS medium was significantly better than the DKW and WPM medium (P < 0.05), due to the fact that in MS medium embryos had the highest height and a better proportion of stem/root to obtain complete plantlets. After this, seedlings were adapted to environmental conditions (greenhouse).
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