MORPHOLOGICAL FEATURES OF SPERMATOZOA FROM MALE WHITE RATS UNDER EXPERIMENTAL STRESS
2021
Inessa Y. Arestova | Evgeniya G. Sharonova | Marina Y. Kupriyanova
We present the effect of stressogenic factors on the morphological parameters of spermatozoa. Objective. We studied the morpho-physiological features of spermatozoa from male albino rats under experimental stress. Materials and methods. A experiment was carried out using sexually mature outbred white rats. Combined stress was performed to an experimental group: after three days of starvation, an emotional stress of “free swimming in a cage” technique was provided. Under a microscope, the spermatozoa were counted per volume of epididymal suspension, the activity was evaluated, and the morphology was studied. Results. A study of the epididymal suspension of male rats under starvation and “emotional” stress showed a decrease in the total number of spermatozoa and sperm motility indices, and an increase in the abnormal gamete forms compared to the control. Conclusion. The combined effect of even short-term stressogenic factors leads to significant changes in the spermogram, allowing the quantitative and qualitative seminal fluid parameters to be a convincing criterion of maladaptation processes in the body under the stress.
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