MICROPROPAGAÇÃO DE Mimosa caesalpiniaefolia Benth. A PARTIR DE SEGMENTOS NODAIS E ÁPICES CAULINARES

Francisco Fábio Mesquita Oliveira; Kathia Maria Barbosa e Silva; Goretti Fernandes de Oliveira; Iron Macêdo Dantas; Ramiro Gustavo Valera Camacho

MICROPROPAGAÇÃO DE Mimosa caesalpiniaefolia Benth. A PARTIR DE SEGMENTOS NODAIS E ÁPICES CAULINARES

2007

Francisco Fábio Mesquita Oliveira | Kathia Maria Barbosa e Silva | Goretti Fernandes de Oliveira | Iron Macêdo Dantas | Ramiro Gustavo Valera Camacho

Mimosa caesalpiniaefolia Benth., (Mimosaceae) it´s a Northeast native species from Brazil and cause its multiple utilities, it has been intensively used. Is conventionally propagated through seeds, but this process isn't easy and plant with a wide range of genetic variability. For this reason, the micropropagation assumes importance. Two experiments had been lead, being that in the first, different combination of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzilaminopurine (BAP) were evaluated and in the second, two ways of culture. In the first experiment the explants had been gotter from three years old plants and inoculated with MS medium (Murashige & Skoog, 1962) substance in different concentrations of 2,4-D and BAP. The observed variables were number of shoots for explants, number sprouted explants and percentile of contamination. In the second experiment explants from sprouted in vitro plantlets inoculated with a complementary formularization substances called MC1 (25 mL of medium with next composition: 20% water coconut (v/v), myo-inositol (0,1 g.L-1), ascorbic acid (0,09g.L-1)) and MC2 (20% water coconut (v/v), myo-inositol (0,1 g.L-1), ascorbic acid (0,1 g.L-1), (0,0155 g.L-1), tiamin (0,02 g.L-1), boron (0,0015 g.L-1), extract of malt (0,3 g.L-1)), both solidificed with agar (7g.L-1). In these experiments the observed variables were number of shoots per treatment and number of callus per treatment. In the combination of 2,4-D and BAP, none morphogenetic reply in the inoculeted tissues was identified. The substances MC1 and MC2 had induced in average callus sprouted in 85,8% of the inoculeted explants. In the first experiment, 34% were infested by fungus.

Show more [+]