STUDY OF SOME FACTORS AFFECT ON CLONAL PROPAGATION FOR "GARDENIA" PLANT Gardenia jasminoides VAR. VEITCHII BY TISSUE CULTURE
2013
Ammar Zeki Kassab Bashi
This experiments were conducted to study the effect of some important factors on growth and multiplication of ornamental plant dendritic "Gardenia" Gardenia Jasminoides var. Veitchii culturing on MS medium In vitro. The study includes the effect of explant position on shoot (Apical shoot , 1st , 2nd , 3rd and 4th node), concentration of Myo-inositol (50 , 75 , 100 , 125 and 150 mg/L), pH of medium (5 , 5.2 , 5.4 , 5.6 , 5.8 and 6), Type of chelate Iron with its concentration (Fe-EDTA contains 5.6 mg/L Fe and Fe-EDDHA contains 5.6 , 11.2 , 16.8 and 22.4 mg/L Fe) in addition to Agar concentrations (4 , 5, 6, 7 and 8 g/L). After 8 weeks from culturing, the results revealed predominant of the 2nd node significantly over the apical shoot and 1st node in number of shoots / explant. The largest number of shoots / node and the number of the shoots longer than 0.5 cm / node was formed in the media which contain 125 mg / L myo-inositol. The medium with PH 6 was highly significant in comparison with other media in different pH , in number of shoots and number of shoots longer than 0.5 cm / node. The chelate ferrous Fe-EDDHA differed significantly from Fe-EDTA in all the studied traits for all treatments, which showed significant increase at concentration of 11.2 mg/L, while the best plant height and of shoots longer than 0.5 cm was appeared when applied agar at concentration 5 g/L, but the concentration 4 g/L Agar as well caused the largest number of shoots and significantly dominant over the agar concentration 7 and 8 g / L.
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