The impact of light spectral composition on the in vitro growth of summer savory (Satureja hortensis L.) plants
2019
D. A. Khlebnikova | A. A. Lobova | O. N. Aladina | M. Yu. Cherednichenko
Relevance. Summer savory (Satureja hortensis L.) is an annual herbaceous plant whose essential oil and extracts are used in medicine, perfumery and the food industry.Methods. The article presents the results of studying the effect of the ratio of blue (BL) and red light (RL) in the total spectrum of LED lamps on the morphometric parameters of plants of summer savory varieties Gnom and Perechny aromat in vitro. For in vitro culture, seeds were sterilized with 5% NaCl solution for 10 minutes, placed in Petri dishes with Murashige and Skoog culture medium (MS). Aseptic seedlings at the age of 4-5 days were transplanted into tubes and transferred to racks with LED lamps (for 28 days): (1) from 1st day to 14th day 100% RL, from the 15th to the 28th day 60% RL, 40% BL; (2) 90% RL, 10% BL; (3) 60% RL and 40% BL; (4) 32% RL and 68% BL. The total illumination intensity in all variants was 175 μmol/m2 s.Results. It was found that a change in the RL:BL ratio in the total spectrum affects the morphometric parameters of the savory plants in an in vitro culture. When cultivated under 100% RL, tall plants with long internodes and small leaves are formed, a reduction in the share of RL to 60...32% results in more short-growing plants with large leaves. The results of this work can be used to develop the technology of clonal micropropagation of valuable breeding forms, as well as to create source material for breeding by in vitro culture methods.
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