ESTANDARIZACIÓN DE LA EXTRACCIÓN DE ADN GENÓMICO EN Tabebuia rosea (Bertol.) DC. Y Cordia alliodora (Ruiz & Pav.) Okén
2012
Paola A. López-Mora | Ana M. López-Gutiérrez | Marta L. Marulanda-Ángel
Because of their great commercial potential and its role on environmental protection, Cordia alliodora and Tabebuia rosea are among species prioritized by Colombia’s National Reforestation Plan, which is executed by the National Corporation for Forestry Research and Development (CONIF) and the Ministry of Agriculture and Rural Development (MADR). Thanks to the advancement of molecular biology techniques, new tools, that are quite useful in plant breeding, are now available, promoting the fixation of genes involved in the expression of desirable phenotypic traits of commercial and productive interest. This study was aimed to standardize a specific genomic DNA extraction method from fresh and dry leaf tissue as well as from seedlings. Significant differences were observed between both species. In C. alliodora, tissue, type and condition was not a limiting factor for extraction and it only depended on the method used. On the other hand, T. rosea has limitations in DNA extraction and amplification from dry tissue, which was attributed to the high concentration of polysaccharides, polyphenols and other secondary metabolites, also reported in other studies for this species. The extracted DNA was quantified and its quality evaluated by DNA amplification using microsatellites. Data were analyzed by both Principal Component Analysis (PCA) and Multiple Factor Analysis (MFA). Based on the results obtained, a DNA extraction protocol was standardized for each of these species and the optimum leaf tissue for that process was defined.
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