STANDARDIZA TION OF ENZYME LINKED IMMUNOSORBENT ASSAY FOR DETERMINATION OF SEROLOGICAL RESPONSE OF CHICKENS TO HYDROPERICARDIUM SYNDROME VACCINE
2002
Kamal.T.. K. Naeeml. P. Das and A. Rahiml
The present study was conducted to standardize the Enzyme Linked Immunosorbent Assay (ELISA) for the assessment of serological response of vaccinated chicks with avian adenovirus-4 (AA V-4 ) .The AA V-4 was grown on chicken embryo liver cell culture after plaque purification. The stock virus was utilized to standardize, ELISA and preparation of experimental oil-based hydropericardium syndrome vaccine (OBHPSV) and aqueous base hydropericardium syndrome vaccine (ABHPSV). Optimum ELISA titres (87.16) were recorded for 1:64 dilution of antigen 1:80 dilution of test serum and 1:1000 dilution of conjugate. Serological response of chicks vaccinated at different age groups was monitored using ELISA and it was interred that vaccination at 3rd day of age with OBHPSV and at 10th day of age with ABHPSV gave maximum titers.
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