Assessment of corn starch as substitute for agarose in DNA gel electrophoresis
2021
Francis Tanam Djankpa | Gideon Akuamoah Wiafe | Bernard Ntim Boateng | Korantema Mawuena Tsegah | Samuel Essien-Baidoo | Mark Bilinyi Ulanja | Kwame Ofori Affram | Abdala Mumuni Ussif | Desmond Owusu Agyeman | Gabriel Asante
Abstract Objective The use of agarose in nucleic acid electrophoresis is the gold standard. However, agarose is very expensive and not readily available in resource limited developing countries like Ghana. Hence, finding a more affordable and readily available alternative to agarose will be a major boost to molecular research in developing countries. This study was aimed at investigating the use of corn starch as a potential substitute for agarose in DNA gel electrophoresis. Results Genomic deoxyribonucleic acid (DNA) extracted from Plasmodium falciparum and primers were obtained from the West African Centre for Cell Biology of Infectious Pathogens and amplified using polymerase chain reaction. The amplicon was run on agarose gel to ascertain the molecular weight (as a positive control). When visualized under both blue light and ultraviolet light, the DNA and ladder showed clear and clean bands with the expected molecular weight. Corn starch was then modified with sodium borate buffer, casted into a gel and used to run the same DNA sample. Our findings indicated that similar to agarose, the DNA sample and ladder migrated successfully through the modified starch gel but no bands were visible when visualized under blue and ultra-violet light.
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