Intracellular Staining for Phosphorylated STAT4 and STAT5 in Mouse Splenocytes
2014
Ana Villegas-Mendez | J. de Souza | Seen-Wai Lavelle | Emily Findlay | Tovah Shaw | Christiaan Saris | Christopher Hunter | Eleanor Riley | Kevin Couper
The Stat (Signal Transducer and Activator of Transcription) family of proteins are critical signal transducers involved in fundamental cellular processes, including cell growth and differentiation, development, apoptosis, immune responses and inflammation. In here, we describe a simple and reproducible flow cytometry protocol to measure Stat protein phosphorylation in splenocyte preparations from malaria infected mice.
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