Characterization of the High-Quality Genome Sequence and Virulence Factors of <i>Fusarium oxysporum</i> f. sp. <i>vasinfectum</i> Race 7
2024
Dingyi Yang | Xiaojun Zhang | Yuqing Ming | Chenglin Liu | Xianlong Zhang | Shiming Liu | Longfu Zhu
<i>Fusarium oxysporum</i> f. sp. <i>vasinfectum</i> (<i>Fov</i>) is a common soilborne fungal pathogen that causes Fusarium wilt (FW) disease in cotton. Although considerable progress has been made in cotton disease-resistance breeding against FW in China, and the <i>R</i> gene conferring resistance to <i>Fov</i> race 7 (FOV) in Upland cotton (<i>Gossypium hirsutum</i>) has been identified, knowledge regarding the evolution of fungal pathogenicity and virulence factors in <i>Fov</i> remains limited. In this study, we present a reference-scale genome assembly and annotation for FOV7, created through the integration of single-molecule real-time sequencing (PacBio) and high-throughput chromosome conformation capture (Hi-C) techniques. Comparative genomics analysis revealed the presence of six supernumerary scaffolds specific to FOV7. The genes or sequences within this region can potentially serve as reliable diagnostic markers for distinguishing <i>Fov</i> race 7. Furthermore, we conducted an analysis of the xylem sap proteome of FOV7-infected cotton plants, leading to the identification of 19 proteins that are secreted in xylem (<i>Fov</i>SIX). Through a pathogenicity test involving knockout mutants, we demonstrated that <i>FovSIX16</i> is crucial for the full virulence of FOV7. Overall, this study sheds light on the underlying mechanisms of <i>Fov</i>’s pathogenicity and provides valuable insights into potential management strategies for controlling FW.
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