<i>Bacillus subtilis</i> Supplementation in a High-Fat Diet Modulates the Gut Microbiota and Ameliorates Hepatic Lipid Accumulation in Grass Carp (<i>Ctenopharyngodon idella</i>)
2022
Daoyuan Guo | Mengqi Xie | Hang Xiao | Lili Xu | Shiyu Zhang | Xiaoxuan Chen | Zhixin Wu
To study the effects of <i>Bacillus subtilis</i> supplementation in a high-fat diet on the gut microbiota and nonalcoholic fatty liver disease in grass carp (<i>Ctenopharyngodon idella</i>), juveniles (60 ± 5 g) were fed three diets: (a) a control diet (CON), (b) a high-fat diet (HFD) and (c) a high-fat diet supplemented with <i>B. subtilis</i> (HFD + BS). After 8 weeks of feeding, fish growth, serum biochemical indices and total liver lipid content were measured, and gut microbiota analysis was performed using the MiSeq250 high-throughput sequencing platform. The results of this study showed that <i>B. subtilis</i> could improve growth and blood serum indices and reduce lipid deposition in the fish liver, preventing fatty liver disease. A grass carp model of fatty liver induced by a high-fat diet was successfully established. Moreover, <i>B. subtilis</i> altered the intestinal microbiota of HFD-fed grass carp, making it more similar to that of the control group. This study revealed the important effects of <i>B. subtilis</i> on grass carp with fatty liver induced by a high-fat diet and provides the foundation for the application of probiotics in grass carp farming.
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