Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
2019
Kathryn M Tabor | Gregory D Marquart | Christopher Hurt | Trevor S Smith | Alexandra K Geoca | Ashwin A Bhandiwad | Abhignya Subedi | Jennifer L Sinclair | Hannah M Rose | Nicholas F Polys | Harold A Burgess
Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.
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