Study of the catalytic properties of bacillus subtilis proteases Estudio de las propiedades catalíticas de las proteasas bacillus subtilis
1998
Salcedo L. | Castellanos O. | Grebeshova R.
The catalytic properties of proteases isolated from the filtrate of submerged fermentation of Bacillus subtilis were investigated. Proteases present in the filtrate were determined to be of the serine protease type based on the use of specific protease inhibitors; ethylenediamintetraacetic acid (EDTA) was used as a metalloprotease inhibitor, and phenylmethylsulfonylfluoride (PMSF) was used as a serine protease inhibitor. Protease activity was highly stable in alkaline solutions and at high temperatures as well as in the presence of detergents. We propose that this protease preparation be used as biocomponent in detergent production.<br><span style="line-height: 115%; font-family: ";Arial";,";sans-serif";; font-size: 12pt; mso-ansi-language: ES-AR; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold; mso-fareast-theme-font: minor-latin;" lang="ES-AR">Se investigaron las propiedades catalíticas de las proteasas obtenidas del filtrado de cultivo de la bacteria Bacillus subtilis. Utilizando inhibidores específicos de proteasas se determinó que las proteasas presentes en el filtrado pertenecían al grupo de las serina proteasas. Se utilizó ácido etilendiaminatetraacético (EDTA) como inhibidor de metaloproteasas, y fenilmetilsulfonil fluoruro (FMSF) como inhibidor de serina proteasas. La actividad proteolítica fue altamente estable en soluciones alcalinas y a altas temperaturas, además tolero la presencia de detergentes. Se propone que estas proteasas sean utilizadas en calidad de biocomponente para la producción de detergentes.</span>
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