Isolation and Characterization of Microsatellite Markers for <i>Canavalia cathartica</i> and <i>C. lineata</i> (Fabaceae)
2013
Asuka Yamashiro | Tadashi Yamashiro | Yoichi Tateishi
Premise of the study: Microsatellite markers were developed for the sea-dispersed legume <i>Canavalia cathartica</i> to investigate its genetic diversity and population structure. We also applied these microsatellite markers to the closely related species <i>C. lineata</i>. Methods and Results: Ten primer sets were developed for <i>C. cathartica</i>, and all of the primers were amplified in <i>C. lineata</i>. The number of alleles per locus ranged from two to 13 and one to 10 for <i>C. cathartica</i> and <>C. lineata</i>, respectively. The expected heterozygosity ranged from 0.375 to 0.870 and from 0.071 to 0.877 for <i>C. cathartica</i> and <i>C. lineata</i>, respectively. Conclusions: The 10 microsatellite markers developed in this study can be used to analyze the population genetic structure of <i>C. cathartica</i> and other related taxa.
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