A Convenient Plant-Based Detection System to Monitor Androgenic Compound in the Environment
2019
Dong-Gwan Kim | Ramin Bahmani | Jae-Heung Ko | Seongbin Hwang
Environmental androgen analogues act as endocrine disruptors, which inhibit the normal function of androgen in animals. In the present work, through the expression of a chimeric gene specified for the production of the anthocyanin in response to androgen DHT (dihydrotestosterone), we generated an indicator <i>Arabidopsis</i> that displays a red color in leaves in the presence of androgen compounds. This construct consists of a ligand-binding domain of the human androgen receptor gene and the poplar transcription factor gene <i>PtrMYB119</i>, which is involved in anthocyanin biosynthesis in poplar and <i>Arabidopsis</i>. The transgenic <i>Arabidopsis</i> XVA-<i>PtrMYB119</i> displayed a red color in leaves in response to 10 ppm DHT, whereas it did not react in the presence of other androgenic compounds. The transcript level of <i>PtrMYB119</i> peaked at day 13 of DHT exposure on agar media and then declined to its normal level at day 15. Expressions of anthocyanin biosynthesis genes including chalcone flavanone isomerase, chalcone synthase, flavanone 3-hydroxylase, dihydroflavonol 4-reductase, UFGT (UGT78D2), and anthocyanidin synthase were similar to that of <i>PtrMYB119</i>. It is assumed that this transgenic plant can be used by nonscientists for the detection of androgen DHT in the environment and samples such as food solution without any experimental procedures.
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