Efficacy of Cymbopogon khasianus essential oil infused chitosan nanoemulsion for mitigation of Aspergillus flavus and aflatoxin B1 contamination in stored Syzygium cumini seeds
2024
Jitendra Prasad | Monisha Soni | Arati Yadav | Kishor Kumar Paul | Manish Kumar Jha | Umakant Banjare | Somenath Das | Nawal Kishore Dubey | Abhishek Kumar Dwivedy
This research developed a novel chitosan nanoemulsion infused with Cymbopogon khasianus essential oil (CKEO-ChNe) and characterized through Dynamic light scattering, Atomic force microscopy, scanning electron microscopy, fourier transform infrared spectroscopy, and x-ray diffractometry analyses followed by its efficacy testing against fungi and aflatoxin B1 contamination in Syzygium cumini seeds. The CKEO-ChNe inhibited Aspergillus flavus and aflatoxin B1 (AFB1) production at 0.32 and 0.28 µL/mL with enhanced antioxidant activity and controlled delivery strategies. The inhibition of methylglyoxal and ergosterol biosynthesis, leakage of ions and molecular interaction of geraniol with Ver-1 (Versicolorin A dehydrogenase) and Omt-A (O-methyl transferase) proteins suggested the antifungal and anti-AFB1 mechanism of action. Further, the in situ protection of Syzygium cumini seeds against fungi, AFB1 contamination and lipid peroxidation (p<0.05) without altering the sensory characteristics, and favorable safety profile in mammalian model recommend the potentiality of encapsulated CKEO nanoemulsion as smart nano-fungitoxic preservative in agricultural and pharmaceutical industries.
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