Fine Mapping of <i>fw6.3</i>, a Major-Effect Quantitative Trait Locus That Controls Fruit Weight in Tomato
2023
Yu Ning | Kai Wei | Shanshan Li | Li Zhang | Ziyue Chen | Feifei Lu | Pei Yang | Mengxia Yang | Xiaolin Liu | Xiaoyan Liu | Xiaotian Wang | Xue Cao | Xiaoxuan Wang | Yanmei Guo | Lei Liu | Xin Li | Yongchen Du | Junming Li | Zejun Huang
Tomato (<i>Solanum lycopersicum</i>) is a widely consumed vegetable, and the tomato fruit weight is a key yield component. Many quantitative trait loci (QTLs) controlling tomato fruit weight have been identified, and six of them have been fine-mapped and cloned. Here, four loci controlling tomato fruit weight were identified in an F<sub>2</sub> population through QTL seq.; <i>fruit weight 6.3 (fw6.3</i>) was a major-effect QTL and its percentage of variation explanation (R<sup>2</sup>) was 0.118. This QTL was fine-mapped to a 62.6 kb interval on chromosome 6. According to the annotated tomato genome (version SL4.0, annotation ITAG4.0), this interval contained seven genes, including <i>Solyc06g074350</i> (the <i>SELF-PRUNING</i> gene), which was likely the candidate gene underlying variation in fruit weight. The <i>SELF-PRUNING</i> gene contained a single-nucleotide polymorphism that resulted in an amino acid substitution in the protein sequence. The large-fruit allele of <i>fw6.3</i> (<i>fw6.3<sub>HG</sub></i>) was overdominant to the small-fruit allele <i>fw6.3<sub>RG</sub></i>. The soluble solids content was also increased by <i>fw6.3<sub>HG</sub></i>. These findings provide valuable information that will aid the cloning of the <i>FW6.3</i> gene and ongoing efforts to breed tomato plants with higher yield and quality via molecular marker-assisted selection.
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