Expression, purification, and bioactivity of a soluble recombinant ovine interferon-tau in Escherichia coli

Yu Hai-Yang; Gao Dong-Mei; Zhou Wei; Xia Bing-Bing; He Zhi-Yuan; Wu Bo; Jiang Min-Zhi; Wang Ming-Li; Zhao Jun

Expression, purification, and bioactivity of a soluble recombinant ovine interferon-tau in Escherichia coli

2021

Ovine interferon-tau (oIFN-τ) is a newly discovered type I interferon. This study used biochemical techniques to transform the oIFN-τ gene into Escherichia coli to obtain the mass and soluble expression of the recombinant protein.

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purification

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Published in

Journal of Veterinary Research

Volume 65 Issue 1 Pagination 101 - 108 ISSN 2450-8608

Publisher

Sciendo

Other Subjects

Ovine interferon-tau; Soluble expression; Cytopathic effect inhibition assay; Antiviral activity

Language

English

In AGRIS since: 2024-12-12

Format: DOAJ

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DOI https://doi.org/10.2478/jvetres-2021-0011

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Expression, purification, and bioactivity of a soluble recombinant ovine interferon-tau in Escherichia coli

2021

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Bibliographic information