Determination of the 1L-5-6L MGF110 Genes Influence on the Biological Properties of the African Swine Fever Virus (Asfarviridae; Asfivirus) “Volgograd/14c” in vivo
2024
Vlasov Mikhail E. | Nefedeva Mariia V. | Kudryashov Dmitriy A. | Titov Ilya A.
African swine fever continues to pose a global agricultural problem due to the absence of vaccine prevention and the high cost of anti-epizootic measures. This study examines the functional role of the African swine fever virus (ASFV) 1L-5-6L multigene family 110 genes in vivo. Four clinically healthy Large White pigs were used in this study. Two groups of animals were inoculated with either the parental strain or the deletion variant, respectively. For subsequent challenge infection, the homologous virulent strain “Stavropol 01/08” was used. Blood samples were collected at specific time intervals. The ASFV infectious activity was determined by titration in porcine blood-derived macrophages. Virus-specific antibodies to the ASFV p30 protein were detected using an enzyme-linked immunosorbent assay (ELISA).
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