In Vitro Propagation of Lemon Citrus lemon local using WPM media
2024
Irfan Shukor
This research was conducted in the Plant Tissue Culture Laboratory in the Department of Horticulture and Landscape Design/College of Agriculture/University of Kirkuk-IRAQ for period from March (2023) to April (2024) to propagate local lemon (Citrus lemon local.) In vitro. During the sterilization stage, the effect of NaOCl was tested at concentrations (2 - 4 - 6) % with duration (2 - 4 - 6) minutes. The proliferation and multiplication stage used different concentrations of BA (0.0, 0.5, 1.0, 1.5, 2.0) mg L-1 and Kin (0.0, 2.0, 4.0, 6.0, 8.0) mg L-1 for WPM media. in the multiplication stage after eight weeks, the highest average number of branches was 4.60 branches. Plant part-1 and the highest average number of leaves was 17.10 leaves. Plant part-1 on. The percentage of chlorophyll in the leaves of plants grown in WPM media supplemented with BA was measured, where the highest wavelength at the chlorophyll level was (0.1971) nm in the media supplemented with 1.5 mg. L-1, while the highest wavelength at the level of chlorophyll b was (1.810) nm in a media supplemented with 0.5 mg. L-1, while the highest total wavelengths were (1.970) nm in the media supported with 0.5 mg. L-1, while in the media supported by Kin, where the highest wavelength was at the level a (4.490) nm in the media supplied with 8.0 mg. L-1, while the highest wavelength at the b level (19.74) nm in the center of the supply was 6.0 mg. L-1, where the highest total wavelength (21.80) nm at the center of the supply was 6.0 mg. L-1, where the leaves collected the cotyledons in the multiplication stage 8 weeks after planting. The resulting vegetative growths were used in the multiplication stage in the process of in vitro micrografting on bitter orange seed stock.
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