Identification of two plus trees of Larix gmelinii var. japonica based on DNA information and its application
2019
Recently, the hybrid (Fsub(1)) larch of Larix gmelinii var. japonica crossed with Japanese larch L. kaempferi has maintained its status as an important breeding species in Hokkaido, and superior hybrid larch derived from the specific plus trees of L. gmelinii var. japonica has been developed. For facilitating tree breeding programs and maintaining seed orchard, we genetically identified the two specific plus trees (Nakashibetsu5 and Nakashibetsu3) and their progeny by using molecular markers. Genotyping analysis successfully distinguished two types of trees and discriminate the candidate parent of progeny. In addition, it was found that clone trees of 'Nakashibetsu5' and that of 'Nakashibetsu3' planted in Forestry Research Institute, Hokkaido Research Organization had the genotype of 'Nakashibetsu5', and a few of clone trees had different genotypes probably resulting from defective management. Further application of molecular information would be required for the broad and careful assessment of the breeding resources in Larix and other woody species.
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