Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks | Estabelecimento de um índice de patogenicidade em cepas de salmonella enteritidis e salmonella typhimurium inoculadas em pintos de um dia de idade
2016
Lima, Daiane Alves de | Furian, Thales Quedi | Pilatti, Roberta Marmitt | Silva, G.L. | Morgam, R.B. | Borges, Karen Apellanis | Fortes, F.B.B. | Moraes, Hamilton Luiz de Souza | Brito, Benito Guimarães de | Brito, Karen Krystine Gonçalves de | Salle, Carlos Tadeu Pippi
Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p<0.05) among the origins of the isolates (p<0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening.
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