Development of a Japanese parrotfish species identification method using PCR-RFLP analysis and its application: Determination of the species composition of parrotfish juveniles in seagrass beds of the Ryukyu Islands, southern Japan
2015
Ogawa, N. (Kochi University, Nankoku, Kochi (Japan). Graduate School of Integrated Arts and Sciences, Department of Agriculture) | Seki, S. | Yamada, H. | Nakamura, Y.
This study aimed to present diagnostic DNA markers for the identification of 34 Japanese parrotfish species (family Scaridae) and to reveal the species composition of parrotfish juveniles in the seagrass beds of the Ryukyu Islands, southern Japan. Using eight restriction enzymes, we determined that the polymerase chain reaction-restriction fragment length polymorphism technique can be used to identify parrotfish species based on the digestion pattern of recognition sites. Applying this technique, we were able to identify seven species (Chlorurus sordidus, Scarus psittacus, S. ghobban, S. rivulatus, S. schlegeli, S. globiceps, and S. quoyi) from 224 parrotfish juveniles collected from seagrass beds in Ishigaki, Okinawa, and Yagaji islands in August and September during 2011-2013. Of these, the first three species were common to all islands, whereas the last two species were found only in Okinawa or Yagaji islands.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Agriculture, Forestry and Fisheries Research Information Technology Center