Detecting endotoxin activity in bovine serum using an automated testing system
2015
Suzuki, K. ((Rakuno Gakuen University, Ebetsu, Hokkaido (Japan). School of Veterinary Medicine), (Ohio State University, Columbus, OH (U.S.A.). College of Veterinary Medical Science)) | Shimamori, T. | Sato, A. | Tsukano, K. | Tsuchiya, M. | Lakritz, J.
The aim of the present study was to compare the ability of the commercially available portable test system (PTSsup(TM)) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80degC for 10 min. The performance of the PTSsup(TM) was not significantly different from that of the traditional LAL-based assays. The results using PTSsup(TM) correlated with those using KT (r**2=0.963, P<0.001) or KC assays (r**2=0.982, P<0.001). Based on these findings, the PTSsup(TM) could be applied as a simplified system to assess endotoxin activity in bovine serum.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Agriculture, Forestry and Fisheries Research Information Technology Center